Optimization of HPLC-tandem mass spectrometry for chlortetracycline using response surface analysis
Optimization of HPLC-tandem mass spectrometry for chlortetracycline using response surface analysis / Hyokwan Bae, Hee-Suk Jung, Jin-Young Jung
p. 309-315 ; 26 cm
수록자료: Environmental engineering research. Korean Society of Environmental Engineers. Vol.23 no.3(2018 Sept.), p. 309-315 23:3<309 ISSN 1226-1025↔ 저자: Hyokwan Bae, Department of Civil and Environmental Engineering, Pusan National University 저자: Hee-Suk Jung, Plant Engineering Division, Institute for Advanced Engineering 저자: Jin-Young Jung, Department of Environmental Engineering, Yeungnam University
Chlortetracycline (CTC) is one of the most important compounds in antibiotic production, and its distribution has been widely investigated due to health and ecological concerns. This study presents systematic approach to optimize the high-performance liquid chromatography–tandem mass spectrometry for analyzing CTC in a multiple reaction monitoring mode (479 → 462 m/z). One-factor-at-a-time (OFAT) test with response surface analysis (RSA) was used as optimization strategies. In OFAT tests, the fragmentor voltage, collision energy, and ratio of acetonitrile in the mobile phase were selected as major factors for RSA. The experimental conditions were determined using a composite in cube design (CCD) to maximize the peak area. As a result, the partial cubic model precisely predicted the peak area response with high statistical significance. In the model, the (solvent composition) and (collision energy²) terms were statistically significant at the 0.1 α-level, while the two-way interactions of the independent variables were negligible. By analyzing the model equation, the optimum conditions were derived as 114.9 V, 15.7 eV, and 70.9% for the fragmentor voltage, collision energy, and solvent composition, respectively. The RSA, coupled with the CCD, offered a comprehensive understanding of the peak area that responds to changes in experimental conditions.