A comparative study of three different viability tests for chemically or thermally inactivated Escherichia coli
A comparative study of three different viability tests for chemically or thermally inactivated Escherichia coli / Seon Yeong Park, Chang Gyun Kim
p. 282-287 ; 26 cm
수록자료: Environmental engineering research. Korean Society of Environmental Engineers. Vol.23 no.3(2018 Sept.), p. 282-287 23:3<282 ISSN 1226-1025↔ 저자: Seon Yeong Park, Department of Environmental Engineering, Inha University 저자: Chang Gyun Kim, Department of Environmental Engineering, Inha University
Three different methods of bacterial viability monitoring were compared to detect chemically or thermally inactivated Escherichia coli. Direct colony enumeration, live/dead bacterial cell staining with a fluorescent dye, and the dehydrogenase activity assay were compared with respect to their ease of use and time required to perform the three different tests. The green (live cell)/red (dead cell) ratio obtained from the fluorescent bacterial cell staining approach showed a linear relationship with the colony forming units; the result obtained with dehydrogenase was similar to those. The sensitivity of the monitoring methods to detect bacterial deactivation varied with different disinfection conditions. After thermal treatment, the sensitivity of the staining approach was lower, while that of the dehydrogenase activity assay was the highest. After chemical treatment, the sensitivity of detection for both methods was similar.