도서
Schizosaccharomyces pombe : methods and protocols
- 표제/저자사항
- Schizosaccharomyces pombe : methods and protocols / edited by Teresa L. Singleton
Singleton, Teresa L.
- 발행사항
- New York : Humana Press, [2018]
©2018
- 형태사항
- xii, 244 pages : illustrations (some color) ; 27 cm
- 총서사항
- (Methods in molecular biology, ISSN 1064-3705 ; 1721)
(Springer protocols)
- 주기사항
- Includes bibliographical references and index
- 표준번호/부호
- ISBN 9781493975457
ISBN 1493975455
ISBN 9781493975464 (eBook)
ISBN 1493975463 (eBook)
ISSN 1940-6029 (electronic)
- 분류기호
- 듀이십진분류법-> 579.563
- 주제명
- Schizosaccharomyces pombe Schizosaccharomyces
- 내용유형
- text
- 매체유형
- unmediated
- 수록매체유형
- volume
CONTENTS
Preface = vii
Contributors = xi
1 Preparation of Solutions and Reagents / Teresa L. Singleton = 1
2 Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale Using DNA Combing / Atanas Kaykov ; Paul Nurse = 9
3 Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChiP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe / Valerie Migeot ; Damien Hermand = 25
4 Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs / Fortunato Ferrara ; Andrew R.M. Bradbury ; Sara D'Angelo = 35
5 Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast / Ge Li ; Richard Y. Zhao = 47
6 Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast / Robert Roth ; Hiten D. Madhani ; Jennifer F. Garcia = 63
7 Analysis of Reverse Transcribed mRNA Using PCR and Polyacrylamide Gel Electrophoresis / Pranjal Biswas ; Uddalak Majumdar ; Sanjay Ghosh = 73
8 The No-Nonsens SDS-PAGE / Lars Backman ; Karina Persson = 89
9 Crystallization of Recombinant ex-Actinin and Related Proteins / Karina Persson ; Lars Backman = 95
10 Estimation of GFP-Nucleoporin Amount Based on Fluorescence Microscopy / Haruhiko Asakawa ; Yasushi Hiraoka ; Tokuko Haraguchi = 105
11 Antibody Pull-Down Experiments in Fission Yeast / Qjanhua Dong ; Fei Li = 117
12 Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation / Akihisa Matsuyama ; Atsuko Shirai ; Minoru Yoshida = 125
13 Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo / Zhen Zhu ; Olivier Frey ; Andreas Hierlemann = 135
14 In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe / Jinpu Yang ; Fei Li = 155
15 High-Frequency Lithium Acetate Transformation of Schizosaccharomyces pombe / Sudhir Kumar Rai ; Angela Atwood-Moore ; Henry L. Levin = 167
16 Tetrad Dissection in Fission Yeast / Wilber Escorcia ; Susan L. Forsburg = 179
17 Random Spore Analysis in Fission Yeast / Wilber Escorcia ; Susan L. Forsburg = 189
18 Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains / Sudhir Kumar Rai ; Angela Atwood-Moore ; Henry L. Levin = 197
19 Schizosaccharomyces pombe Biotechnological Applications in Winemaking / Ángel Benito ; Fernando Calderón ; Santiago Benito = 217
20 Schizosaccharomyces pombe Isolation Protocol / Ángel Benito ; Fernando Calderón ; Santiago Benito = 227
Glossary = 235
Index = 243
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