A novel retron of Vibrio parahaemolyticus is closely related to retron-Vc95 of Vibrio cholerae
A novel retron of Vibrio parahaemolyticus is closely related to retron-Vc95 of Vibrio cholerae / Toshi Shimamoto, Ashraf M. Ahmed, Tadashi Shimamoto
p. 323-328 ; 29 cm
수록자료: The Journal of microbiology. The Microbiological Society of Korea(MSK). Vol.51 no.3(2013 June), p. 323-328 51:3<323 ISSN 1225-8873 저자: Toshi Shimamoto, Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University 저자: Ashraf M. Ahmed, Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University 저자: Tadashi Shimamoto, Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University E-MAIL: firstname.lastname@example.org
Some bacteria produce a satellite RNA-DNA complex termed msDNA, multicopy single-stranded DNA. In this report, msDNA from Vibrio parahaemolyticus, a cause of acute gastroenteritis, was identified and named msDNA-Vp96. The retron element containing the ret gene, encoding the reverse transcriptase (RT) that is responsible for msDNA production, was cloned and characterized. Comparison of msDNA-Vp96 and msDNA-Vc95, from Vibrio cholerae, showed a high level of sequence similarity. We exchanged the two ret genes to examine whether msDNA was produced by the RT from different sources. We found that RT-Vp96 of V. parahaemolyticus was able to synthesize msDNA-Vc95 of V. cholerae and vice versa. To the best of our knowledge, this is the first report that RT from different bacterial species can synthesize msDNA.