Development of diagnostic and vaccine markers through cloning, expression, and regulation of putative virulence-protein-encoding genes of Aeromonas hydrophila
Development of diagnostic and vaccine markers through cloning, expression, and regulation of putative virulence-protein-encoding genes of Aeromonas hydrophila / Vijai Singh, Dharmendra Kumar Chaudhary, Indra Mani, Rohan Jain, B.N. Mishra
p. 275-282 ; 29 cm
수록자료: The Journal of microbiology. The Microbiological Society of Korea(MSK). Vol.51 no.3(2013 June), p. 275-282 51:3<275 ISSN 1225-8873 저자: Vijai Singh, Molecular Diagnostics and Biotechnology Laboratory, Division of Crop Protection Central Institute for Subtropical Horticulture ; National Bureau of Fish Genetic Resource E-MAIL: firstname.lastname@example.org 저자: Dharmendra Kumar Chaudhary, National Bureau of Fish Genetic Resources 저자: Indra Mani, National Bureau of Fish Genetic Resources ; Department of Biochemistry, Faculty of Science, Banaras Hindu University 저자: Rohan Jain, Department of Environmental Biotechnology, UNESCO-IWE 저자: B.N. Mishra, Department of Biotechnology, Institute of Engineering and Technology, Uttar Pradesh Technical University
Aeromonas hydrophila is an opportunistic bacterial pathogen that is associated with a number of diseases in fish, amphibians, reptiles, and humans. In fish it causes several disease symptoms including tail and skin rot, and haemorrhagic septicemia; in human it causes soft-tissue wound infection and diarrhoea. The pathogenesis of A. hydrophila is multifactorial, but the mechanism is unknown so far. It is considered to be mediated by expression and secretion of extracellular proteins such as aerolysin, lipase, chitinase, amylase, gelatinase, hemolysins, and enterotoxins. A number of the putative virulence-protein-encoding genes that are present in the genome of A. hydrophila have been targeted by PCR for molecular diagnosis. These significant genes are also targeted for over-production of proteins by cloning and expression methods. In this review, we emphasize recent progress in the cloning, expression, and regulation of putative virulence-protein-encoding genes of A. hydrophila for a better understanding of the pathogenesis and also help to provide effective strategies for control of diseases.