Intracellular polysaccharide and its antioxidant activity by Pleurotus citrinopileatus SM-01
표제/저자사항
Intracellular polysaccharide and its antioxidant activity by Pleurotus citrinopileatus SM-01 / Su-Qian Wu, Shang-Long Gao, Hong-Hong Liu, Xin-Yi Sun, Long Hao, Le Jia, Li-Fei Pang, Shou-Hua Jia, Meng-Shi Jia
형태사항
p. 660-668 ; 29 cm
주기사항
수록자료: Macromolecular research. Polymer Society of Korea. Vol.21 no.6(2013 June), p. 660-668 21:6<660 ISSN 1598-5032 저자: Su-Qian Wu, College of Life Science, Shandong Agricultural University 저자: Shang-Long Gao, College of Life Science, Shandong Agricultural University 저자: Hong-Hong Liu, College of Life Science, Shandong Agricultural University 저자: Xin-Yi Sun, College of Life Science, Shandong Agricultural University 저자: Long Hao, College of Life Science, Shandong Agricultural University 저자: Le Jia, College of Life Science, Shandong Agricultural University E-MAIL: jiale9015@163.com 저자: Li-Fei Pang, College of Chemistry and Material Science, Shandong Agricultural University 저자: Shou-Hua Jia, College of Chemistry and Material Science, Shandong Agricultural University E-MAIL: shjia@sdau.edu.cn 저자: Meng-Shi Jia, The Second High School of Taian
The extraction parameters of intracellular polysaccharide (IPS) from Pleurotus citrinopileatus SM-01 mycelia were optimized, and the in vitro and in vivo antioxidant activities of IPS were investigated. The optimum conditions of IPS extraction were predicted to be an ultrasonic treatment time of 664.09 s, precipitation time of 23.03 h and pH 7.36, and IPS yield was estimated at 16.13%. The in vitro inhibition effects of IPS at a dosage of 5 g/L on the superoxide anion, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals were 73.96±4.62%, 69.2±4.37%, and 50.75±4.39%, respectively, which were 72.56±5.08%, 22.83±1.94%, and 43.93±3.26% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of IPS was 0.9±0.07, 69.81±5.24% higher than that of BHT. The activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and alanine aminotransferase (ALT) in mice blood were 241.38±23.19, 454.95±42.39, 60.32±5.16, and 32.39±2.54 U/mL, respectively, and the malonaldehyde (MDA) level was 9.54±0.72 nmol/mL. The results provided a reference for the large-scale extraction of IPS by P. citrinopileatus SM-01 in industrial fermentation, suggesting that the IPS can be used as a potential antioxidant, which enhances adaptive immune responses.
