The genetic variation and intraspecific relationships between 35 cultivars of Lactuca sativo were investigated using external morphology and Randomly Amplified Polymorphic DNA (RAPD) by random docamer and simple "ence repeat (SSR) primers. The 12 qualitative characters from the seed, stem, head type, leaf and anlhoayanin were examihed for 35 cultivars. The seed color was classified into two types; brown and black. Stem fasciation was observed in Celtitce, Feria, and Petite Rouge. Head formation was divided into two types such as open and closed, and 29 cultivars were not formated. Leaves were also grouped into several types based on the overall shape, margin, color, surface profile, presence or absence of lobe, venation, glossiness, and anthocyanin color and its distribution. The two leaf characters such as surfkce profile and venation were shown to be the same patterns within all of the analyzed Chima cufivars. The 37 primers out of 113 random and SSR primers were amplified for all tested plants. The 230 (87.1%) among 264 bands derived from 37 primers showed polymorphism, and 6.2 bands per primer were observed. The polymorphic bands of random decamer and SSR primers were 152 (87.9%) and 78 (85.7%), respectively. Numbers of bands per primer were 3 to 13, and the average number was 7.1. Similarity indices for cluster analysis were ranged from 0.70 to 0.95. The phenograms for 35 analyzed cultivars by RAPD markers were discordance with the result of morphological classifications by 12 qualitative characters.